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Authors' reply
2 Department of Pathology, K.S. Hegde Medical Academy, Mangalore, Karnataka, India
3 Department of Microbiology, K.S. Hegde Medical Academy, Mangalore, Karnataka, India
Correspondence Address:
Manjunath M Shenoy
Department of Skin and V.D., Justice K.S. Hegde Charitable Hospital, Deralakatte, Mangalore - 574 160 Karnataka
India
How to cite this article: Shenoy MM, Teerthanath S, Karnaker VK, Girisha B S, Prasad K, Pinto J. Authors' reply. Indian J Dermatol Venereol Leprol 2009;75:73-74 |
Sir,
We thank Singh and Lavanya for showing interest and responding to our article "Comparison of potassium hydroxide mount and mycological culture with histopathologic examination using periodic acid-Schiff staining of the nail clippings in the diagnosis of onychomycosis". [1] We agree with some of the views expressed, however others may need reconsideration.
It is true that mycological culture has a higher specificity, but it has lower sensitivity as compared to histopathology with periodic acid-Schiff staining (HP/PAS). [1],[2] HP/PAS is relatively quick, and a specificity of 72% cannot be overlooked. [3] In most cases, morphological aspect of the hyphae and/or spores can suggest the group to which pathogens belong. [3] In personal view of the authors, cost of HP/PAS is not so high considering all these informations obtained after doing this test, and when compared to the cost of onychomycosis therapy.
HP/PAS is not an alternate test to mycologic culture. It a simple and highly sensitive screening test and can be performed as a complementary to mycological culture and potassium hydroxide (KOH) mount to close the diagnostic gap. [1] PAS staining can be performed routinely as a standard of practice in the lab diagnosis of onychomycosis, however, histochemical staining using Gomori methenamine silver may have higher sensitivity. [4] All these factors suggest that HP/PAS staining of nail clips should be considered as a valuable test in the diagnosis of onychomycosis.
1. |
Shenoy MM, Teerthanath S, Karnaker VK, Girisha BS, Krishna Prasad MS, Pinto J. Comparison of potassium hydroxide mount and mycological culture with histopathologic examination using periodic acid-Schiff staining of the nail clippings in the diagnosis of onychomycosis. Indian J Dermatol Venereol Leprol 2008;74:226-9.
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2. |
Weinberg JM, Koestenblatt EK, Tutrone WD, Tishler HR, Najarian L. Comparison of diagnostic methods in the evaluation of onychomycosis. J Am Acad Dermatol 2003;49:193-7.
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Gianni C, Morelli V, Cerri A, Greco C, Rossini P, Guiducci A, et al. Usefulness of histological examination for the diagnosis of onychomycosis. Dermatology 2001;202:283-8.
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D'Hue Z, Perkins SM, Billings SD. GMS is superior to PAS for diagnosis of onychomycosis. J Cutan Pathol 2008;35:745-7.
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