Using tuberculin syringe for withdrawing fibrin-plug in platelet-rich fibrin (PRF) therapy

Senkadhir Vendhan; Shekhar Neema; Biju Vasudevan; Senkadhirdasan Dakshinamurthy

doi:10.25259/IJDVL_1115_2023

View/Download PDF

Buy Reprints

PDF

Translate this page into:

Pearls

90 (

5

); 692-693

doi:

10.25259/IJDVL_1115_2023

pmid:

38841976

Using tuberculin syringe for withdrawing fibrin-plug in platelet-rich fibrin (PRF) therapy

Senkadhir Vendhan^,, Shekhar Neema¹, Biju Vasudevan, Senkadhirdasan Dakshinamurthy²

Department of Dermatology, Armed Forces Medical College, Wanowrie, Pune, Maharashtra, India

1Department of Dermatology, Command Hospital Kolkata, West Bengal, India

2Department of Community Medicine, Mahatma Gandhi Medical College and Research Institute, Puducherry, India

Corresponding author: Dr. Senkadhir Vendhan, Department of Dermatology, Armed Forces Medical College, Wanowrie, Pune, Maharashtra, India. vendhan100@gmail.com

Received: 2023-10-21, Accepted: 2023-12-06, Epub ahead of print: 2024-04-16, Published: 2024-08

Licence

This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-Share Alike 4.0 License, which allows others to remix, transform, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

How to cite this article: Vendhan S, Neema S, Vasudevan B, Dakshinamurthy S. Using tuberculin syringe for withdrawing fibrin-plug in platelet-rich fibrin (PRF) therapy. Indian J Dermatol Venereol Leprol. 2024;90:692-3. doi: 10.25259/IJDVL_1115_2023

Problem

Platelet-rich fibrin (PRF) therapy is a useful treatment option for a variety of non-healing ulcers. PRF secretes a number of growth factors such as platelet-derived growth factor, transforming growth factor beta 1, vascular endothelial growth factor and insulin-like growth factors, as well as cytokines and matrix glycoproteins such as thrombospondin 1, fibronectin and vitronectin.¹ One of the logistic challenges encountered during the procedure is removing the fibrin plug from the test tube. Often, it is impossible to fit commonly available non-toothed dissecting forceps into the tube; hence small-sized forceps are utilised to remove the plug. Even with the small forceps, it is difficult to grip the fibrin plug as it slides quickly and falls back into the tube, making the process tedious.

Solution

To address this issue, we propose extracting the fibrin plug from the tube with a one-ml tuberculin syringe. Blood is collected in a sterile test tube and spun for 10 minutes in a centrifuge at 3000 revolutions per minute (RPM). When platelet-rich fibrin is ready, a one-ml tuberculin syringe without a needle is introduced into the test tube, and once in contact with the fibrin plug, suction is applied on the syringe and it is withdrawn from the tube and placed over the wound [Figure 1]. Because a suction mechanism with a syringe is used, the entire platelet-rich fibrin plug comes out quickly and without deformation [Figure 2 and Video 1]. This procedure can simplify and expedite the entire process.

Tuberculin syringe used to pull out the fibrin plug from the test tube.

Video 1: Demonstration of platelet-rich fibrin plug withdrawal using tuberculin syringe in a lepromatous neuropathic ulcer.

Download

Declaration of patient consent

Patient consent not required as there are no patients in this study.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.

Use of artificial intelligence (AI)-assisted technology for manuscript preparation

The authors confirm that there was no use of artificial intelligence (AI)-assisted technology for assisting in the writing or editing of the manuscript and no images were manipulated using AI.