Generic selectors
Exact matches only
Search in title
Search in content
Post Type Selectors
Search in posts
Search in pages
Filter by Categories
15th National Conference of the IAOMFP, Chennai, 2006
Abstract
Abstracts from current literature
Acne in India: Guidelines for management - IAA Consensus Document
Addendum
Announcement
Art & Psychiatry
Article
Articles
Association Activities
Association Notes
Award Article
Book Review
Brief Report
Case Analysis
Case Letter
Case Letters
Case Notes
Case Report
Case Reports
Clinical and Laboratory Investigations
Clinical Article
Clinical Studies
Clinical Study
Commentary
Conference Oration
Conference Summary
Continuing Medical Education
Correspondence
Corrigendum
Cosmetic Dermatology
Cosmetology
Current Best Evidence
Current Issue
Current View
Derma Quest
Dermato Surgery
Dermatopathology
Dermatosurgery Specials
Dispensing Pearl
Do you know?
Drug Dialogues
e-IJDVL
Editor Speaks
Editorial
Editorial Remarks
Editorial Report
Editorial Report - 2007
Editorial report for 2004-2005
Errata
Erratum
Focus
Fourth All India Conference Programme
From Our Book Shelf
From the Desk of Chief Editor
General
Get Set for Net
Get set for the net
Guest Article
Guest Editorial
History
How I Manage?
IADVL Announcement
IADVL Announcements
IJDVL Awards
IJDVL AWARDS 2015
IJDVL Awards 2018
IJDVL Awards 2019
IJDVL Awards 2020
IJDVL International Awards 2018
Images in Clinical Practice
Images in Dermatology
In Memorium
Inaugural Address
Index
Knowledge From World Contemporaries
Leprosy Section
Letter in Response to Previous Publication
Letter to Editor
Letter to the Editor
Letter to the Editor - Case Letter
Letter to the Editor - Letter in Response to Published Article
LETTER TO THE EDITOR - LETTERS IN RESPONSE TO PUBLISHED ARTICLES
Letter to the Editor - Observation Letter
Letter to the Editor - Study Letter
Letter to the Editor - Therapy Letter
Letter to the Editor: Articles in Response to Previously Published Articles
Letters in Response to Previous Publication
Letters to the Editor
Letters to the Editor - Letter in Response to Previously Published Articles
Letters to the Editor: Case Letters
Letters to the Editor: Letters in Response to Previously Published Articles
Media and news
Medicolegal Window
Messages
Miscellaneous Letter
Musings
Net Case
Net case report
Net Image
Net Images
Net Letter
Net Quiz
Net Study
New Preparations
News
News & Views
Obituary
Observation Letter
Observation Letters
Oration
Original Article
ORIGINAL CONTRIBUTION
Original Contributions
Pattern of Skin Diseases
Pearls
Pediatric Dermatology
Pediatric Rounds
Perspective
Presedential Address
Presidential Address
Presidents Remarks
Quiz
Recommendations
Regret
Report
Report of chief editor
Report of Hon : Treasurer IADVL
Report of Hon. General Secretary IADVL
Research Methdology
Research Methodology
Resident page
Resident's Page
Resident’s Page
Residents' Corner
Residents' Corner
Residents' Page
Retraction
Review
Review Article
Review Articles
Reviewers 2022
Revision Corner
Self Assessment Programme
SEMINAR
Seminar: Chronic Arsenicosis in India
Seminar: HIV Infection
Short Communication
Short Communications
Short Report
Snippets
Special Article
Specialty Interface
Studies
Study Letter
Study Letters
Supplement-Photoprotection
Supplement-Psoriasis
Symposium - Contact Dermatitis
Symposium - Lasers
Symposium - Pediatric Dermatoses
Symposium - Psoriasis
Symposium - Vesicobullous Disorders
SYMPOSIUM - VITILIGO
Symposium Aesthetic Surgery
Symposium Dermatopathology
Symposium-Hair Disorders
Symposium-Nails Part I
Symposium-Nails-Part II
Systematic Review and Meta-Analysis
Systematic Reviews and Meta-analyses
Systematic Reviews and Meta-analysis
Tables
Technology
Therapeutic Guideline-IADVL
Therapeutic Guidelines
Therapeutic Guidelines - IADVL
Therapeutics
Therapy
Therapy Letter
Therapy Letters
View Point
Viewpoint
What’s new in Dermatology
Generic selectors
Exact matches only
Search in title
Search in content
Post Type Selectors
Search in posts
Search in pages
Filter by Categories
15th National Conference of the IAOMFP, Chennai, 2006
Abstract
Abstracts from current literature
Acne in India: Guidelines for management - IAA Consensus Document
Addendum
Announcement
Art & Psychiatry
Article
Articles
Association Activities
Association Notes
Award Article
Book Review
Brief Report
Case Analysis
Case Letter
Case Letters
Case Notes
Case Report
Case Reports
Clinical and Laboratory Investigations
Clinical Article
Clinical Studies
Clinical Study
Commentary
Conference Oration
Conference Summary
Continuing Medical Education
Correspondence
Corrigendum
Cosmetic Dermatology
Cosmetology
Current Best Evidence
Current Issue
Current View
Derma Quest
Dermato Surgery
Dermatopathology
Dermatosurgery Specials
Dispensing Pearl
Do you know?
Drug Dialogues
e-IJDVL
Editor Speaks
Editorial
Editorial Remarks
Editorial Report
Editorial Report - 2007
Editorial report for 2004-2005
Errata
Erratum
Focus
Fourth All India Conference Programme
From Our Book Shelf
From the Desk of Chief Editor
General
Get Set for Net
Get set for the net
Guest Article
Guest Editorial
History
How I Manage?
IADVL Announcement
IADVL Announcements
IJDVL Awards
IJDVL AWARDS 2015
IJDVL Awards 2018
IJDVL Awards 2019
IJDVL Awards 2020
IJDVL International Awards 2018
Images in Clinical Practice
Images in Dermatology
In Memorium
Inaugural Address
Index
Knowledge From World Contemporaries
Leprosy Section
Letter in Response to Previous Publication
Letter to Editor
Letter to the Editor
Letter to the Editor - Case Letter
Letter to the Editor - Letter in Response to Published Article
LETTER TO THE EDITOR - LETTERS IN RESPONSE TO PUBLISHED ARTICLES
Letter to the Editor - Observation Letter
Letter to the Editor - Study Letter
Letter to the Editor - Therapy Letter
Letter to the Editor: Articles in Response to Previously Published Articles
Letters in Response to Previous Publication
Letters to the Editor
Letters to the Editor - Letter in Response to Previously Published Articles
Letters to the Editor: Case Letters
Letters to the Editor: Letters in Response to Previously Published Articles
Media and news
Medicolegal Window
Messages
Miscellaneous Letter
Musings
Net Case
Net case report
Net Image
Net Images
Net Letter
Net Quiz
Net Study
New Preparations
News
News & Views
Obituary
Observation Letter
Observation Letters
Oration
Original Article
ORIGINAL CONTRIBUTION
Original Contributions
Pattern of Skin Diseases
Pearls
Pediatric Dermatology
Pediatric Rounds
Perspective
Presedential Address
Presidential Address
Presidents Remarks
Quiz
Recommendations
Regret
Report
Report of chief editor
Report of Hon : Treasurer IADVL
Report of Hon. General Secretary IADVL
Research Methdology
Research Methodology
Resident page
Resident's Page
Resident’s Page
Residents' Corner
Residents' Corner
Residents' Page
Retraction
Review
Review Article
Review Articles
Reviewers 2022
Revision Corner
Self Assessment Programme
SEMINAR
Seminar: Chronic Arsenicosis in India
Seminar: HIV Infection
Short Communication
Short Communications
Short Report
Snippets
Special Article
Specialty Interface
Studies
Study Letter
Study Letters
Supplement-Photoprotection
Supplement-Psoriasis
Symposium - Contact Dermatitis
Symposium - Lasers
Symposium - Pediatric Dermatoses
Symposium - Psoriasis
Symposium - Vesicobullous Disorders
SYMPOSIUM - VITILIGO
Symposium Aesthetic Surgery
Symposium Dermatopathology
Symposium-Hair Disorders
Symposium-Nails Part I
Symposium-Nails-Part II
Systematic Review and Meta-Analysis
Systematic Reviews and Meta-analyses
Systematic Reviews and Meta-analysis
Tables
Technology
Therapeutic Guideline-IADVL
Therapeutic Guidelines
Therapeutic Guidelines - IADVL
Therapeutics
Therapy
Therapy Letter
Therapy Letters
View Point
Viewpoint
What’s new in Dermatology
View/Download PDF

Translate this page into:

Letter to the Editor
2014:80:1;78-80
doi: 10.4103/0378-6323.125475

Malassezia associated hyperkeratosis of the nipple in young females: Report of three cases

Conghui Li1 , Yuping Ran2 , Takashi Sugita3 , Enshi Zhang3 , Zhen Xie2 , Ludan Cao2
1 Department of Dermatovenereology, West China Hospital, Sichuan University; The Second People's Hospital of Chengdu City, Jinjiang, Chengdu, Sichuan, China
2 Department of Dermatovenereology, West China Hospital, Sichuan University, Chengdu, Sichuan, China
3 Department of Microbiology, Meiji Pharmaceutical University, Kiyose, Tokyo, Japan

Correspondence Address:
Yuping Ran
Department of Dermatovenereology, West China Hospital, Sichuan University, 37 Guoxue Road, Chengdu - 610041, Sichuan Province
China
How to cite this article:
Li C, Ran Y, Sugita T, Zhang E, Xie Z, Cao L. Malassezia associated hyperkeratosis of the nipple in young females: Report of three cases. Indian J Dermatol Venereol Leprol 2014;80:78-80
Copyright: (C)2014 Indian Journal of Dermatology, Venereology, and Leprology

Sir,

Hyperkeratosis of the nipple and/or areola (HNA) is an unusual condition characterized by hyperpigmented, verrucous, keratotic thickening of the nipple and/or areola. [1] We report three cases of hyperkeratosis of the nipple associated with Malassezia.

Case 1

A 21-year-old woman had suffered a recurrent papule and effusion on her right nipple [Figure - 1]a for more than 15 years. There was no pain, and the papule was not itchy. The patient had taken advice from a gynecologist and had been followed up for 7 years. However, since no improvement was noted, the gynecologist did a biopsy of the nipple considering breast cancer. Her family history was negative, and the patient had no history of ichthyosis or eczema. A skin examination showed crusting and effusion on the right nipple.

Figure 1: Lesions on the right nipple of patient 1 (a) and on both nipples in patients 2 and 3 (b and c)

Case 2

A 19-year-old woman with asymptomatic verrucous lesions on both nipples for 3 years was referred to the clinic. The lesions consisted of a round, rough plaque, 2 cm in diameter, on each nipple. They showed dark-brown discoloration and a cobbled hyperkeratotic appearance [Figure - 1]b. The patient was otherwise healthy and had no other skin lesions.

Case 3

A 16-year-old girl presented with a 1-year history of brown patches associated with pain and itching on her nipples. On examination, we found brown, waxy scales on both nipples [Figure - 1]c. The patient′s nipples were inverted. There was no history of eczema, Darier′s disease, or ichthyosis.

Biopsies performed in patients 1 and 3 revealed papillomatous elongation of the epidermis with hyperkeratosis. A number of budding yeast cells were detected in the stratum corneum by periodic acid-Schiff (PAS) and Gomori methenamine silver (GMS) staining [Figure - 2].

Figure 2: Budding yeast cells were detected in the stratum corneum in cases 1 (a and b, ×400) and 3 (c and d, ×400) by using periodic acid-Schiff (PAS) and Gomori methenamine silver (GMS) staining

Direct methylene blue staining of scrapings revealed yeast cells in all three patients [Figure - 3]. Scales were cultured and the colonies examined under a microscope, which revealed yeast cells. Three strains recovered from the medium were identified as Malassezia species. Fungal DNA was extracted from paraffin-embedded tissues of patients 1 and 3, and from scales of patient 2, according to the method of Sugita et al. [2] Overall colonization by all Malassezia species and colonization by M. globosa and M. restricta were determined by real-time polymerase chain reaction (PCR) analysis of the samples, performed according to the method of Sugita et al. [2] All samples were analyzed in triplicate. M. globosa was detected in the samples from patients 1, 2, and 3 in all three cases. M. restricta was detected in patient 2.

Figure 3: Direct methylene blue staining of scrapings revealed yeast cells in all three patients

The first patient was treated with oral itraconazole (0.2 g, twice a day) and topical povidone-iodine for 16 days [Figure - 4]a. The other two patients finally improved after treatment with oral itraconazole (0.2 g, twice a day), topical 1% naftifine-0.25% ketoconazole cream, and washing with 2% ketoconazole lotion for 14 days [Figure - 4]b and c, and no yeasts were found by direct mycological examination. No recurrence has been observed in any of the three patients till date.

Figure 4: All three patients improved after treatment for 14 days

The three patients were female, in the age group 16-21 years. None of them had pityriasis versicolor or seborrheic dermatitis. Females in this age range often have high levels of estrogen secretion, but endocrinological investigations showed that the levels of female hormones in the three patients were within normal limits. Doppler ultrasonograms of the breasts were normal. We found no evidence that the cases we reported were connected with the endocrine system. While terra firma-forme dermatosis and dermatosis neglecta have similar clinical manifestations, such pigmented plaques could be easily removed using isopropyl alcohol. [3] In contrast, repeated efforts to scrub the affected area with different types of soaps and cleaners, as well as isopropyl alcohol, failed to improve the condition. It is rare that hyperkeratosis of the nipple is associated with Malassezia infection. Malassezia sp. are lipophilic yeasts of the cutaneous microbiota and have been implicated in various diseases, including pityriasis versicolor, seborrheic dermatitis, Malassezia folliculitis, atopic dermatitis, and psoriasis. [4] Taizo et al. reported a case of confluent and reticulate papillomatosis with Malassezia sp. in the lesion. [5] Tamraz et al. reviewed 10 cases of confluent and reticulated papillomatosis, and observed Malassezia yeast in six cases. An improper keratinizing response to Malassezia has been hypothesized as underlying the pathological keratinization. [6] A similar improper keratinizing process may be underlying the three cases reported here. Boralevi et al. reported two cases of hyperkeratotic head and neck Malassezia dermatosis; M. globosa and M. sympodialis were identified from the scrapings. [7] In the three cases reported here, M. globosa were dominant in cases 1 and 3, but M. restricta was detected in case 2. It is still unclear whether the different species are related to body regions or ethnic or geographic background.

In conclusion, Malassezia yeasts were detected in the lesion and antifungal therapy was effective in all these patients; therefore, we consider that Malassezia might be a new factor associated with hyperkeratosis of the nipple. Although it is still unsettled whether Malassezia is the direct cause, antifungal therapy could be considered besides keratolytic therapy, cryotherapy, and retinoid therapy in patients with hyperkeratosis of the nipple.

Acknowledgment

We thank Dr. Xinyu Lin for providing case 3.

References
1.
Shastry V, Betkerur J, Kushalappa PA. Unilateral nevoid hyperkeratosis of the nipple: A report of two cases. Indian J Dermatol Venereol Leprol 2006;72:303-5.
[Google Scholar]
2.
Sugita T, Suto H, Unno T, Tsuboi R, Ogawa H, Shinoda T, et al. Molecular analysis of Malassezia microflora on the skin of atopic dermatitis patients and healthy subjects. J Clin Microbiol 2001;39:3486-90.
[Google Scholar]
3.
Lucas JL, Brodell RT, Feldman SR. Dermatosis neglecta: A series of case reports and review of other dirty-appearing dermatoses. Dermatol Online J 2006;12:5.
[Google Scholar]
4.
Gupta AK, Batra R, Bluhm R, Boekhout T, Dawson TL Jr. Skin diseases associated with Malassezia species. J Am Acad Dermatol 2004;51:785-98.
[Google Scholar]
5.
Hamaguchi T, Nagase M, Higuchi R, Takiuchi I. A case of confluent and reticulated papillomatosis responsive to ketoconazole cream. Nihon Ishinkin Gakkai Zasshi 2002;43:95-8.
[Google Scholar]
6.
Tamraz H, Raffoul M, Kurban M, Kibbi AG, Abbas O. Confluent and reticulated papillomatosis: Clinical and histopathological study of 10 cases from Lebanon. J Eur Acad Dermatol Venereol 2013;27:e119-23.
[Google Scholar]
7.
Boralevi F, Marco-Bonnet J, Lepreux S, Buzenet C, Couprie B, Taïeb A. Hyperkeratotic head and neck Malassezia dermatosis. Dermatology 2006;212:36-40.
[Google Scholar]

Fulltext Views
3,131

PDF downloads
2,365
Show Sections